pmk-t vector Search Results


plasmid vector pmkt  (Toyobo)
98
Toyobo plasmid vector pmkt
<t>A.</t> <t>DNA</t> regions (Upstream and Downstream) corresponding to the target gene are amplified by PCR with the specific primer sets having the 20 bp homologous sequence for the <t>pMKT</t> (shown as red, blue, green and yellow). B. Digestion of the pMKT vector with StuI enzyme generates 2 DNA fragments named Digested vectors L and S after their lengths (L-long; S-short). The red hatched areas indicate the homologous repeat sequence (i.e., URA5.3 terminator region). C. Four DNA fragments (Digested vector L, Digested vector S, Upstream and Downstream) are connected in one reaction tube using the In-fusion system, to make a plasmid pMKT-UPandDOWN). D. The DNA fragment for transformation is amplified by PCR with the primer set UF and DR using pMKT-UPandDOWN as the template.
Plasmid Vector Pmkt, supplied by Toyobo, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmk-t+vector/KOD+-Plus-+Neo/pmc07854268-51-114-132
Average 98 stars, based on 1 article reviews
plasmid vector pmkt - by Bioz Stars, 2026-09
98/100 stars
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pmk-tymv 3'utr (plasmid #123576)  (Addgene inc)
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Standard format: Plasmid sent in bacteria as agar stab
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pmk-tmv 3'utr (plasmid #123575)  (Addgene inc)
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Standard format: Plasmid sent in bacteria as agar stab
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pmk-t7g10 leader (plasmid #123548)  (Addgene inc)
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Standard format: Plasmid sent in bacteria as agar stab
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Image Search Results


A. DNA regions (Upstream and Downstream) corresponding to the target gene are amplified by PCR with the specific primer sets having the 20 bp homologous sequence for the pMKT (shown as red, blue, green and yellow). B. Digestion of the pMKT vector with StuI enzyme generates 2 DNA fragments named Digested vectors L and S after their lengths (L-long; S-short). The red hatched areas indicate the homologous repeat sequence (i.e., URA5.3 terminator region). C. Four DNA fragments (Digested vector L, Digested vector S, Upstream and Downstream) are connected in one reaction tube using the In-fusion system, to make a plasmid pMKT-UPandDOWN). D. The DNA fragment for transformation is amplified by PCR with the primer set UF and DR using pMKT-UPandDOWN as the template.

Journal: Bio-protocol

Article Title: Multiple Modification of Chromosomal Loci Using URA5.3 Selection Marker in the Unicellular Red Alga Cyanidioschyzon merolae

doi: 10.21769/BioProtoc.3204

Figure Lengend Snippet: A. DNA regions (Upstream and Downstream) corresponding to the target gene are amplified by PCR with the specific primer sets having the 20 bp homologous sequence for the pMKT (shown as red, blue, green and yellow). B. Digestion of the pMKT vector with StuI enzyme generates 2 DNA fragments named Digested vectors L and S after their lengths (L-long; S-short). The red hatched areas indicate the homologous repeat sequence (i.e., URA5.3 terminator region). C. Four DNA fragments (Digested vector L, Digested vector S, Upstream and Downstream) are connected in one reaction tube using the In-fusion system, to make a plasmid pMKT-UPandDOWN). D. The DNA fragment for transformation is amplified by PCR with the primer set UF and DR using pMKT-UPandDOWN as the template.

Article Snippet: list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Pipette tips (NIPPON Genetics Co., Ltd., catalog numbers: FG-102, FG-301, FG-401) Toothpicks Cuvettes (As One Corporation., catalog number: 1-2855-02) 1.5 ml Microtube (BM Equipment Co., Ltd., catalog number: NT-175) 50 ml Centrifuge tube (Corning Incorporated, catalog number: 352196) 24-well tissue culture test plate (BM Equipment Co., Ltd., catalog number: 92424) Sterilized plastic plate (Φ 90 mm) (As One Corporation, catalog number: GD90-15) AnaeroPouch (Mitsubishi Gas, catalog number: 2-3764-02) AnaeroPack (Mitsubishi Gas, catalog number: 2-3765-01) C. merolae T1 strain ( Taki et al. , 2015 ) Escherichia coli DH5α (Takara Bio, catalog number: 9057) Wild type C. merolae DNA Salmon sperm DNA (FUJIFILM Wako Pure Chemical Industries Corporation, catalog number: 047-17322) Plasmid vector pMKT ( Takemura et al. , 2018 ; available upon request to authors) KOD-Plus-Neo DNA polymerase (Toyobo, catalog number: KOD-401) GoTaq DNA polymerase (Promega, catalog number: M7123) Stu I (NEB, catalog number: R0187S) Dpn I (Takara Bio, catalog number: 1235A) Specific primers [set for amplifying the Upstream and Downstream fragments with the homologous sequence for the Stu I-digested pMKT (see ), ordered at Integrated DNA Technologies (IDT) Inc.] fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 2. caption a7 caption a8 Overview of the template vector (pMKT-UPandDOWN) and DNA constructs for transformation.

Techniques: Amplification, Sequencing, Plasmid Preparation, Transformation Assay